Simple Colorimetry of Dehydrogenase Activity in Oyster using Tetrazolium Chloride
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چکیده
منابع مشابه
Colorimetry of diaphorase in commercial preparations and clinical chemical reagents by use of tetrazolium salts.
We describe a procedure for assay of diaphorase activity in commercial purified preparations and in clinical chemical reagents by use of iodonitrotetrazolium chloride or other tetrazolium salts. The method is based on measurement of the formazan produced by enzymic reduction of tetrazolium salts in the presence of NADH. The assay procedure has been optimized for linear kinetics, simplicity of o...
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heteropoly acids (hpa) and their salts have advantages as catalysts which make them both economically and environmentally attractive, strong br?nsted acidity, exhibiting fast reversible multi-electron redox transformations under rather mild conditions, very high solubility in polar solvents, fairly high thermal stability in the solid states, and efficient oxidizing ability, so that they are imp...
15 صفحه اولSuccinate Dehydrogenase Activity Assay in situ with Blue Tetrazolium Salt in Crabtree-Positive Saccharomyces cerevisiae Strain
A spectrophotometric method for determining succinate dehydrogenase (SDH) activity assay in azide-sensitive yeast Saccharomyces cerevisiae has been developed. The permeabilization of yeast cells by 0.05 % digitonin permitted to study yeast enzymatic activity in situ. The reduction of blue tetrazolium salt (BT) to blue tetrazolium formazan (BTf) was conducted in the presence of phenazine methosu...
متن کاملDetection of rough dissociants of Pasteurella pestis with tetrazolium chloride.
Lederberg (J. Bact., 56, 695, 1948) reported the use of triphenyl tetrazolium chloride to detect fermentative variants of Ewherichia coli K-12. We have found that smooth and rough colonies of Pasteurella pestis could be identified easily when grown on tryptose (Difco) agar containing this compound. On many routine and modified laboratory media that did not contain tetrazolium, differentiation w...
متن کاملSimple colorimetry of glycated serum protein in a centrifugal analyzer.
A recently described (Clin Chim Acta 127: 87-95, 1982) colorimetric assay for glycated proteins in serum exploits their ketoamine activity to reduce 3,3'-(3,3'-dimethoxy-4,4'-biphenylylene)bis [2-(p-nitrophenyl)-5-phenyl-2H-tetrazolium chloride] (nitroblue tetrazolium) in alkaline solution; the authors termed this the "fructosamine assay." The method is simple, requiring only the addition of on...
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ژورنال
عنوان ژورنال: Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi)
سال: 1985
ISSN: 0015-6426,1882-1006
DOI: 10.3358/shokueishi.26.229